Journal: Kidney international
Article Title: The Inducible Deletion of Drosha and MicroRNAs In Mature Podocytes Results In A Collapsing Glomerulopathy
doi: 10.1038/ki.2011.122
Figure Lengend Snippet: Immunofluorescence of histologic sections from WT and Drosha mutants at 2 and 6 weeks of age stained with antibodies to (i) nephrin (D–F) and (ii) podocin (D–F). Sections were also stained with anit-ZO1 to mark adherent junctions on podocytes (i) A–C and (ii) A–C.
Article Snippet: 41 Antibodies The antibodies used in these studies were: anti-smooth muscle actin (clone 1A4, Sigma), anti-synaptopodin (clone G1D4, Progen Biotechnik), anti-WT-1 (Santa Cruz Biotechnology, Santa Cruz, Ca), anti-desmin (clone D33, Dako, Carpenteria, Ca), anti-nestin (clone 2Q178, Santa Cruz Biotechnology, Santa Cruz, Ca), and anti-ZO1, Alexa Fluor 594 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse Ig (Invitrogen, Carlsbad, CA).
Techniques: Immunofluorescence, Staining